Uncoupling diffusion and binding in FRAP experiments
نویسندگان
چکیده
منابع مشابه
Diffusion in macromolecular crowded media . Monte Carlo simulation of obstructed diffusion vs . FRAP experiments
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A procedure for a systematic evaluation of fluorescence recovery after photo-bleaching (FRAP) data is presented that allows one to determine distributions of diffusion coefficients. The method provides a straightforward and calibration-free way to quantify multiple diffusion processes when FRAP is measured as a function of time and space by means of confocal laser scanning microscopy. It is ver...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2009
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth0309-183a